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GLP-1 Protocol: Advanced

Full Recomposition with Anabolic Protection

Who This Is For

Experienced users who want:

  • Maximum fat loss while preserving or building lean mass.
  • Targeted visceral fat reduction.
  • Deep recomposition: looking bigger while weighing less.
  • Sustained energy and recovery despite aggressive deficit.

This tier adds Tesamorelin for anabolic signaling and visceral fat targeting. Optional additions—AOD-9604 and Ipamorelin—provide further lipolytic and GH support for those who need it.

The Stack

CompoundRole
RetatrutideMetabolic controller: intake, partitioning, oxidative drive
NAD+Energy infrastructure: redox balance, ATP production
L-CarnitineFat transport: shuttles fatty acids into mitochondria
MOTS-cMitochondrial expansion: biogenesis, metabolic flexibility
TesamorelinAnabolic preservation: GH pulsatility, visceral fat targeting, recovery

Optional additions:

CompoundRole
AOD-9604Lipolytic amplifier: accelerates stubborn subcutaneous fat loss
IpamorelinGH secretagogue: supports natural GH pulse; gentler alternative to Tesamorelin

Dosing Protocol

Core Stack

Retatrutide

ParameterSpecification
Dose3–4 mg weekly (some respond to 5–6 mg)
RouteSubcutaneous
FrequencyWeekly or split
NoteIf escalating from Intermediate, allow 4+ weeks before increasing

NAD+

ParameterSpecification
Dose200–300 mg per injection
Frequency5–6×/week or EOD
RouteIM preferred
TimingMorning; may split AM/PM on heavy training days

L-Carnitine

ParameterSpecification
Dose500–1000 mg per injection
FrequencyDaily or 5–6 days/week
TimingFasted morning or 30–45 min pre-workout
RouteIM

MOTS-c

ParameterSpecification
Dose5–10 mg per injection
Frequency2–3×/week
TimingMorning or pre-training, fasted
RouteSubcutaneous
Cycle6–8 weeks on, 2–4 weeks off

Tesamorelin

ParameterSpecification
Dose1–2 mg nightly
Timing30–60 min before sleep, at least 2 hours after last meal
RouteSubcutaneous
MonitoringCheck IGF-1 at week 4–6 and monthly thereafter

Optional Additions

AOD-9604

ParameterSpecification
Dose250–500 mcg per injection
FrequencyDaily (5–6 days/week)
TimingMorning, fasted
RouteSubcutaneous; optional site-specific injection near problem areas
When to addStubborn subcutaneous fat that isn't responding to core stack

Ipamorelin

ParameterSpecification
Dose100–300 mcg per injection
Frequency1–2× daily (commonly before bed and/or AM)
RouteSubcutaneous
When to addAlternative to Tesamorelin if seeking gentler GH support; can combine at lower doses

Why This Combination

The Intermediate stack—Retatrutide, NAD+, L-Carnitine, MOTS-c—maximizes fat oxidation capacity. But deep recomposition requires one more element: an anabolic signal that tells the body what to preserve while fat burns.

Tesamorelin provides that signal. It's a GHRH analog that triggers natural, pulsatile growth hormone release during sleep. This matters because:

  1. GH → IGF-1 sustains mTOR signaling in muscle, maintaining protein synthesis even in caloric deficit.
  2. GH-driven lipolysis targets visceral fat specifically—the deep, inflammatory depot most resistant to change.
  3. Collagen synthesis increases, strengthening tendons, ligaments, and connective tissue.
  4. Sleep architecture deepens, anchoring recovery to the hours when GH peaks.

The combination creates a metabolic environment where fat is the preferred fuel and muscle is the protected asset. Users describe looking "bigger while weighing less"—the hallmark of true recomposition.

AOD-9604 adds peripheral lipolytic support. It increases fatty acid release from stubborn subcutaneous depots without systemic GH effects. Useful when certain areas (lower abdomen, flanks) resist the core stack.

Ipamorelin offers a gentler GH secretagogue option. It produces reliable GH pulses without the cortisol or prolactin elevation seen with some other secretagogues. Can be combined with Tesamorelin at reduced doses or used as a standalone alternative.

Weekly Schedule (Example)

DayRetatrutideNAD+L-CarnitineMOTS-cTesamorelinAOD-9604 (opt)
Mon250 mg AM500 mg fasted10 mg AM2 mg bedtime300 mcg fasted
Tue250 mg AM500 mg pre-WO2 mg bedtime300 mcg fasted
WedWeekly dose250 mg AM500 mg fasted10 mg AM2 mg bedtime300 mcg fasted
Thu250 mg AM500 mg pre-WO2 mg bedtime300 mcg fasted
Fri250 mg AM500 mg fasted10 mg AM2 mg bedtime300 mcg fasted
SatRest or 250 mgOptional2 mg bedtimeOptional
SunRestRest2 mg bedtimeOptional

Timeline: What to Expect

Weeks 1–4

FocusExpected Changes
GH adaptationSleep deepens within first week; vivid dreams common
Fat lossAccelerates to 2–3 lb/week
RecoveryTraining recovery noticeably faster
WaterTransient GH-related water retention may occur; resolves by week 3–4
MuscleFullness maintained despite deficit

Weeks 5–8

FocusExpected Changes
RecompositionVisible transformation: waist drops, limbs hold or gain size
PerformanceStrength maintained or improved; endurance up
Visceral fatBelt notches move; trunk tightens
EnergyHigher than pre-protocol baseline despite lower calories
SleepDeep, restorative; morning alertness high

Weeks 9–12

FocusExpected Changes
DefinitionMuscle separation apparent; stubborn areas finally yielding
Cumulative20–30 lb fat loss typical; lean mass preserved or slightly gained
Metabolic markersSignificant improvements in glucose, TG, HDL, liver enzymes
Physical"Photoshoot ready" look achievable for many
StateMetabolism feels self-sustaining, not effortful

Lifestyle Foundation

ComponentTarget
Protein1.0–1.2 g/lb body weight; anchor each meal at 30–40 g
Training4–5 days/week; power + hypertrophy hybrid
CardioZone 2 predominates (20–40 min, 3–4×/week); HIIT optional if recovery permits
Sleep7–9 hours; Tesamorelin timing is critical—consistent bedtime matters
Hydration3–4 liters daily; higher with GH-related water shifts

Managing Side Effects

GH-Related (Tesamorelin/Ipamorelin)

IssueManagement
Water retention (weeks 1–3)Transient; ensure adequate potassium (4–5 g/day from diet)
Joint stiffness / hand paresthesiaImproves with movement and hydration; reduce dose temporarily if persistent
Blood glucose elevationMonitor if diabetic; GH can transiently raise fasting glucose

Retatrutide-Related

IssueManagement
Nausea/early satietySmaller protein-first meals; hold dose until resolved
ConstipationFiber + fluids → magnesium citrate at bedtime

MOTS-c/L-Carnitine

IssueManagement
Early fatigue (MOTS-c)Resolves within 1 week; ensure sleep and electrolytes
Injection site sorenessRotate sites; use appropriate needle length for IM

Monitoring

TimepointWhat to Track
BaselineFull panel: CBC, CMP, lipids, fasting glucose/insulin, HbA1c, thyroid, IGF-1
Week 4–6IGF-1 (target 50–100% above baseline, not supraphysiologic), fasting glucose
MonthlyIGF-1 while on Tesamorelin
Week 12Full panel; expect: ↓ glucose, ↓ TG, ↑ HDL, ↑ IGF-1, improved liver enzymes

IGF-1 guidance: Target physiologic elevation (upper normal to moderately elevated). If IGF-1 exceeds 350–400 ng/mL, reduce Tesamorelin dose.

What Comes Next

Maintenance:

  • Reduce Retatrutide to 2–3 mg/week.
  • Tesamorelin to 1 mg nightly for ongoing sleep/connective tissue support.
  • NAD+ at 100–150 mg on training or high-stress days.
  • L-Carnitine as needed pre-training.

Lean-gain phase:

  • Discontinue Retatrutide.
  • Maintain Tesamorelin.
  • Increase calories to slight surplus.
  • Continue NAD+ and L-Carnitine to support performance.
  • Goal: slow, clean accrual of lean mass.

Advanced cycling:

  • MOTS-c: 6–8 weeks on, 2–4 weeks off.
  • Tesamorelin can be run continuously with IGF-1 monitoring.
  • Consider periodizing AOD-9604 around stubborn fat phases.

Contraindications

  • Personal or family history of medullary thyroid carcinoma or MEN2 syndrome (Retatrutide).
  • Active malignancy (GH/IGF-1 axis; MOTS-c).
  • Proliferative diabetic retinopathy (GH can worsen).
  • Pregnancy or breastfeeding.
  • Uncontrolled diabetes (requires close monitoring and medication adjustment).